*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , HeLa treated tunicamycin (4 μg/ml)
Mouse, Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Activating Transcription Factor 4,Creb-2,Creb2,Taxreb67,Txreb,Atf4
Cytoplasm , Cell membrane , Nucleus
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq]
GTX101944 WB Image
ATF4 antibody detects ATF4 protein by western blot analysis. Un-treated (-) and treated (+, 4 μg/ml tunicamycin treatment ) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody (GTX101944) diluted at 1:500.