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ATF4 antibody

Orthogonal
Anti-ATF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30070
Anti-ATF4 antibody used in Western Blot (WB). GTX30070
Anti-ATF4 antibody used in Western Blot (WB). GTX30070

Cat. No. GTX30070

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all ATF4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 4 , CREB-2 , CREB2 , TAXREB67 , TXREB

Cellular Localization

Cytoplasm,Cell membrane,Nucleus

Background

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-ATF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30070

GTX30070 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30070 ATF4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATF4 antibody used in Western Blot (WB). GTX30070

GTX30070 WB Image

Orthogonal

WB analysis of HeLa cells treated by tunicamycin (2 μg/mL) for 8 hours using GTX30070 ATF4 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ATF4 antibody used in Western Blot (WB). GTX30070

GTX30070 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019. PMID: 31443530

REFERENCE

Application Reference

Takumi Yokosawa et al. Genes (Basel) 2020; 11 (12) Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells.
Application : WB
Reactivity : Human
Rojas-Morales P et al. Biomolecules 2019; 9 (9) Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399