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ATF6 antibody [37-1]

Orthogonal
Anti-ATF6 antibody [37-1] used in Western Blot (WB). GTX00686
Anti-ATF6 antibody [37-1] used in Western Blot (WB). GTX00686

Cat. No. GTX00686

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 37-1

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μg ($459)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Product Note

This antibody detects both full-length ATF6 alpha as well as the cleaved ATF6 alpha p50 form.This antibody is less efficient for IP compared to GTX00687
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant ATF6 alpha; (amino-terminal fragment of ATF6 alpha; fused to GST)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Background

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-ATF6 antibody [37-1] used in Western Blot (WB). GTX00686

GTX00686 WB Image

Orthogonal

WB analysis of HeLa cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg

Anti-ATF6 antibody [37-1] used in Western Blot (WB). GTX00686

GTX00686 WB Image

Orthogonal

WB analysis of NIH-3T3 cell lysate using GTX00686 ATF6 antibody [37-1]. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6α is constitutively expressed as pATF6α(P), and converted to pATF6α(N) ( > 50-kDa protein) in ER-stressed cells.
pATF6α(P) : the endogenous precursor ATF6α (~90kDa)
pATF6α(N) : the cleaved product of ATF6α(>50kDa)
Lane 1 : untreated
Lane 2 : 1mM DTT (reducing reagent) for 1 h
Lane 3 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h
Lane 4 : 2 μg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h.
Loading : 50μg

REFERENCE

Clone Reference

Mori K et al. Mol Biol Cell. 2010; 21 (9):1435-8 Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 459