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ATF6 antibody [C3], C-term

KO/KD Orthogonal

Cat. No. GTX104820

Reference 5
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Product Note

This antibody was raised against the C-terminus of human ATF6 alpha, meaning it can only detect the uncleaved form.
Knockdown/Knockout validation was supported by references (PMID:29535165)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240489

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Cellular Localization

Endoplasmic reticulum membrane , Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Nucleus

Background

ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM]

Database

Research Area

REFERENCE

Application Reference

Chen YT et al. J Clin Invest 2021; 131 (5) Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts.
Application : WB
Reactivity : Mouse
Wang TE et al. Antioxidants (Basel) 2020; 9 (8)
Application : WB
Reactivity : Human
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Shih YC et al. Circ Res 2018; (Epub) Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.
Application : WB
Reactivity : Human, Mouse
Weng CY et al. Arch Toxicol 2014; 88 (2) : 213-26 * induces unfolded protein response in vascular endothelial cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

ATF6 antibody [C3], C-term Cat. No. GTX104820

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-ATF6 antibody [C3], C-term used in Western Blot (WB). GTX104820
Date : Anonymous submitted on 15-Dec-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16F10 cells
Amount used : 10μg
Blocking : 0.5% skim milk in TBST, RT, 1Hr
Primary Antibody : 1:3000, °C, Hr
Anti-ATF6 antibody [C3], C-term used in Western Blot (WB). GTX104820
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human cardiac fibroblast
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169