APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Product Note
The immunogen sequence of this antibody is within the cleaved form of ATF6 alpha.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 350-377 amino acids of human ATF6.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
activating transcription factor 6 , ACHM7 , ATF6A
Background
This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
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GTX81096 FACS Image
FACS analysis of K562 cells using GTX81096 ATF6 antibody, Internal.
Top histogram : negative control
Bottom histogram : K562 cells
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GTX81096 WB Image
WB analysis of HeLa whole cell lysate using GTX81096 ATF6 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:16000
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GTX81096 WB Image
WB analysis of A2058 cell lysate (35ug/lane) using GTX81096 ATF6 antibody, Internal.
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GTX81096 IHC-P Image
IHC-P analysis of human brain tissue using GTX81096 ATF6 antibody, Internal.
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REFERENCE
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REVIEW
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