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ATF6 antibody

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31729
Anti-ATF6 antibody used in Western Blot (WB). GTX31729
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729
Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729

Cat. No. GTX31729

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ATF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

MCF7 Cell Slide(GTX25536) , EL4 Cell Lysate(GTX27183)

Product Note

This antibody was raised against the C-terminus of human ATF6 alpha, meaning it can only detect the uncleaved form.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.The immunogen is located within amino acids 470 - 520 of ATF6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887626

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 6 , ACHM7 , ATF6A

Background

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-ATF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31729

GTX31729 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX31729 ATF6 antibody.
Working concentration : 10 μg/ml

Anti-ATF6 antibody used in Western Blot (WB). GTX31729

GTX31729 WB Image

WB analysis of EL4 cell lysate using GTX31729 ATF6 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729

GTX31729 IHC-P Image

IHC-P analysis of human breast tissue using GTX31729 ATF6 antibody.
Working concentration : 20 μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729

GTX31729 IHC-P Image

IHC-P analysis of human breast tissue using GTX31729 ATF6 antibody.
Working concentration : 5 μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729

GTX31729 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX31729 ATF6 antibody.
Working concentration : 20 μg/ml

Anti-ATF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX31729

GTX31729 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX31729 ATF6 antibody.
Working concentration : 5 μg/ml

REFERENCE

Application Reference

Fussi N et al. Cell Death Dis 2018; 9 (7) : 757 Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399