GeneTex
United States (US)

ATF6 antibody

GTX31729_2891_WB_20180221_18121410_262.jpg
GTX31729_1369_IHC-P_20180221_18121410_988.jpg
GTX31729_1370_IHC-P_20180221_18121410_328.jpg
GTX31729_1371_IHC-P_20180221_18121410_545.jpg
GTX31729_309_ICC-IF_20180221_18121410_570.jpg
GTX31729_1372_IHC-P_20180221_18121410_505.jpg

Cat No. GTX31729

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

MCF7 Cell Slide(GTX25536) , EL4 Cell Lysate(GTX27183)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.The immunogen is located within amino acids 470 - 520 of ATF6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Activating Transcription Factor 6,Achm7,Atf6A,Atf6

Background

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Database

DATA IMAGES
GTX31729_2891_WB_20180221_18121410_262.jpg

GTX31729 WB Image

WB analysis of EL4 cell lysate using GTX31729 ATF6 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

GTX31729_1369_IHC-P_20180221_18121410_988.jpg

GTX31729 IHC-P Image

IHC-P analysis of human breast tissue using GTX31729 ATF6 antibody.
Working concentration : 5 μg/ml

GTX31729_1370_IHC-P_20180221_18121410_328.jpg

GTX31729 IHC-P Image

IHC-P analysis of human breast tissue using GTX31729 ATF6 antibody.
Working concentration : 20 μg/ml

GTX31729_1371_IHC-P_20180221_18121410_545.jpg

GTX31729 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX31729 ATF6 antibody.
Working concentration : 5 μg/ml

GTX31729_309_ICC-IF_20180221_18121410_570.jpg

GTX31729 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX31729 ATF6 antibody.
Working concentration : 10 μg/ml

GTX31729_1372_IHC-P_20180221_18121410_505.jpg

GTX31729 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX31729 ATF6 antibody.
Working concentration : 20 μg/ml

REFERENCE
Package List Price ($)
$ 339