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ATG10 antibody

Anti-ATG10 antibody used in Western Blot (WB). GTX129097
Anti-ATG10 antibody used in Immunoprecipitation (IP). GTX129097

Cat. No. GTX129097

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATG10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

human Atg10-transfected 293T cells

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ATG10 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885893

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 10 , APG10 , APG10L , pp12616

Cellular Localization

Cytoplasm

Background

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATG10 antibody used in Western Blot (WB). GTX129097

GTX129097 WB Image

ATG10 antibody detects ATG10 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human ATG10-transfected 293T cells
12 % SDS-PAGE
ATG10 antibody (GTX129097) dilution: 1:5000

Anti-ATG10 antibody used in Immunoprecipitation (IP). GTX129097

GTX129097 IP Image

Immunoprecipitation of ATG10 protein from SKNSH whole cell extracts using 5 μg of ATG10 antibody (GTX129097).
Western blot analysis was performed using ATG10 antibody (GTX129097).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169