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ATG10 antibody

Anti-ATG10 antibody used in Western Blot (WB). GTX31329

Cat. No. GTX31329

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ATG10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

SK-N-SH Cell Lysate(GTX23956)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG10 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ATG10.The immunogen is located within the last 50 amino acids of ATG10.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 10 , APG10 , APG10L , pp12616

Cellular Localization

Cytoplasm

Background

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ATG10 antibody used in Western Blot (WB). GTX31329

GTX31329 WB Image

WB analysis of SK-N-SH cell lysate using GTX31329 ATG10 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399