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ATG101 antibody

Anti-ATG101 antibody used in Western Blot (WB). GTX132435
Anti-ATG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX132435

Cat. No. GTX132435

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATG101 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATG101. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886647

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 101 , C12orf44

Background

Autophagy factor required for autophagosome formation.

Database

Research Area

DATA IMAGES

Anti-ATG101 antibody used in Western Blot (WB). GTX132435

GTX132435 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ATG101 antibody (GTX132435) diluted at 1:1000.

Anti-ATG101 antibody used in IHC (Paraffin sections) (IHC-P). GTX132435

GTX132435 IHC-P Image

ATG101 antibody detects ATG101 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
ATG101 antibody (GTX132435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169