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ATG12 antibody [GT166]

KO/KD Orthogonal
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629815

Cat. No. GTX629815

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT166

Isotype

 IgG2b

Application

 WB, ICC/IF, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATG12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] (GTX629815) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

ATG12 antibody [GT166] detects ATG12 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
ATG12 antibody [GT166] (GTX629815) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

ATG12 antibody [GT166] detects ATG12 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
ATG12 antibody [GT166] (GTX629815) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629815

GTX629815 ICC/IF Image

Orthogonal

ATG12 antibody [GT166] detects ATG12 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG12 protein stained by ATG12 antibody [GT166] (GTX629815) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

REFERENCE

Application Reference

Sara Monaci et al. Front Immunol 2020; 11 : 573646 Hypoxia Shapes Autophagy in LPS-Activated Dendritic Cells.
Application : ICC/IF
Reactivity : Human
Lin TY et al. Autophagy 2019; : 1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.
Application : ICC/IF, PLA
Reactivity : Human
Samuel J Kenny et al. bioRxiv 2019; (Epub) Super-resolution microscopy unveils FIP200-scaffolded, cup-shaped organization of mammalian autophagic initiation machinery.
Application : ICC/IF
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations