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ATG12 antibody

Anti-ATG12 antibody used in Western Blot (WB). GTX124181
Anti-ATG12 antibody used in Western Blot (WB). GTX124181
Anti-ATG12 antibody used in Immunoprecipitation (IP). GTX124181
Anti-ATG12 antibody used in Western Blot (WB). GTX124181
Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181
Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181
Anti-ATG12 antibody used in Western Blot (WB). GTX124181

Cat No. GTX124181

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , Rat brain , ATG12 shRNA-transfected 293T

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171947

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

GTX124181 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

GTX124181 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG12 antibody used in Immunoprecipitation (IP). GTX124181

GTX124181 IP Image

Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 μg of ATG12 antibody (GTX124181).
Western blot analysis was performed using ATG12 antibody (GTX124181) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

GTX124181 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATG12 antibody (GTX124181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATG12 stained by ATG12 antibody (GTX124181) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

GTX124181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ATG12 antibody Cat No. GTX124181

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28134285
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf