GeneTex
United States (US)

ATG12 antibody

KO/KD
GTX124181_41038_WB_R_Brain_18121410_832.jpg
GTX124181_42263_20180420_WB_shRNA_watermark_18121410_320.jpg
GTX124181_42333_20160219_IP_18121410_543.jpg
GTX124181_41038_WB_M_18121410_539.jpg
GTX124181_42333_20151210_WB_18121410_383.jpg
GTX124181_43320_20181221_IHC-P_19022001_354.jpg
GTX124181_42305_20160118_IHC-P_R_18121410_248.jpg

Cat No. GTX124181

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , Rat brain , ATG12 shRNA-transfected 293T

Predict Reactivity

Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Autophagy Related 12,Apg12,Apg12L,Fbr93,Hapg12,Atg12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX124181_41038_WB_R_Brain_18121410_832.jpg

GTX124181 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181_42263_20180420_WB_shRNA_watermark_18121410_320.jpg

GTX124181 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181_42333_20160219_IP_18121410_543.jpg

GTX124181 IP Image

Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 μg of ATG12 antibody (GTX124181).
Western blot analysis was performed using ATG12 antibody (GTX124181).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX124181_41038_WB_M_18121410_539.jpg

GTX124181 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181_42333_20151210_WB_18121410_383.jpg

GTX124181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000.

GTX124181_43320_20181221_IHC-P_19022001_354.jpg

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATG12 stained by ATG12 antibody (GTX124181) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124181_42305_20160118_IHC-P_R_18121410_248.jpg

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATG12 antibody (GTX124181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169