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ATG12 antibody

Anti-ATG12 antibody used in Western Blot (WB). GTX31768
Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX31768
Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX31768

Cat No. GTX31768

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG12 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG12.The immunogen is located within the first 50 amino acids of ATG12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ATG12 antibody used in Western Blot (WB). GTX31768

GTX31768 WB Image

WB analysis of human brain tissue lysate using GTX31768 ATG12 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX31768

GTX31768 IHC-P Image

IHC-P analysis of human brain tissue using GTX31768 ATG12 antibody.
Working concentration : 20 μg/ml

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX31768

GTX31768 IHC-P Image

IHC-P analysis of human brain tissue using GTX31768 ATG12 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339