GeneTex
United States (US)

ATG12 antibody

GTX31769_1416_IHC-P_20180221_18121410_140.jpg
GTX31769_2929_WB_20180221_18121410_820.jpg
GTX31769_330_IHC-P_20180221_18121410_600.jpg

Cat No. GTX31769

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Mouse Heart Tissue Lysate(GTX27932)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG12 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ATG12.The immunogen is located within the first 50 amino acids of ATG12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Autophagy Related 12,Apg12,Apg12L,Fbr93,Hapg12,Atg12

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES
GTX31769_1416_IHC-P_20180221_18121410_140.jpg

GTX31769 IHC-P Image

IHC-P analysis of human brain tissue using GTX31769 ATG12 antibody.
Working concentration : 2.5 μg/ml

GTX31769_2929_WB_20180221_18121410_820.jpg

GTX31769 WB Image

WB analysis of mouse heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31769 ATG12 antibody.
Working concentration : 1 μg/ml

GTX31769_330_IHC-P_20180221_18121410_600.jpg

GTX31769 IHC-P Image

IHC-P analysis of human brain tissue using GTX31769 ATG12 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329