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ATG13 antibody

Anti-ATG13 antibody used in Western Blot (WB). GTX85183
Anti-ATG13 antibody used in IHC (Paraffin sections) (IHC-P). GTX85183
Anti-ATG13 antibody used in IHC (Paraffin sections) (IHC-P). GTX85183

Cat. No. GTX85183

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ATG13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Rat Heart Tissue Lysate(GTX28912)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13.The immunogen is located within amino acids 450 - 500 of ATG13.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726107

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 13 , KIAA0652 , PARATARG8

Cellular Localization

Cytoplasm, cytosol

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

Database

Research Area

DATA IMAGES

Anti-ATG13 antibody used in Western Blot (WB). GTX85183

GTX85183 WB Image

WB analysis of rat heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85183 ATG13 antibody.
Working concentration : 1 μg/ml

Anti-ATG13 antibody used in IHC (Paraffin sections) (IHC-P). GTX85183

GTX85183 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX85183 ATG13 antibody.
Working concentration : 20 μg/ml

Anti-ATG13 antibody used in IHC (Paraffin sections) (IHC-P). GTX85183

GTX85183 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX85183 ATG13 antibody.
Working concentration : 5 μg/ml

REFERENCE

Application Reference

Stanton MJ et al. Cancer Res 2013; 73 (1) : 160-71 Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399