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ATG14 antibody

KO/KD
Anti-ATG14 antibody used in Western Blot (WB). GTX119950
Anti-ATG14 antibody used in Western Blot (WB). GTX119950

Cat. No. GTX119950

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATG14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

Huh7 , HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618570

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 14 , ATG14L , BARKOR , KIAA0831

Background

Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

Database

Research Area

DATA IMAGES

Anti-ATG14 antibody used in Western Blot (WB). GTX119950

GTX119950 WB Image

KO/KD

Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX119950) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATG14 antibody used in Western Blot (WB). GTX119950

GTX119950 WB Image

KIAA0831 antibody detects KIAA0831 protein by Western blot analysis.
A. 20 μg Huh7 whole cell lysate/extract
0 % SDS-PAGE
KIAA0831 antibody (GTX119950) dilution: 1:1500

REFERENCE

Application Reference

JaeYung Lee et al. Int J Mol Sci 2021; 22 (17) ATG101 Degradation by HUWE1-Mediated Ubiquitination Impairs Autophagy and Reduces Survival in Cancer Cells.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169