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ATG14 antibody

Anti-ATG14 antibody used in Western Blot (WB). GTX128166
Anti-ATG14 antibody used in IHC (Paraffin sections) (IHC-P). GTX128166
Anti-ATG14 antibody used in Western Blot (WB). GTX128166

Cat No. GTX128166

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa , HepG2 , 293T , RD , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATG14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 14 , ATG14L , BARKOR , KIAA0831

Background

Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

Database

Research Area

DATA IMAGES

Anti-ATG14 antibody used in Western Blot (WB). GTX128166

GTX128166 WB Image

ATG14 antibody detects ATG14 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
ATG14 antibody (GTX128166) dilution: 1:1000

Anti-ATG14 antibody used in IHC (Paraffin sections) (IHC-P). GTX128166

GTX128166 IHC-P Image

ATG14 antibody detects ATG14 protein at cytosol on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
ATG14 antibody (GTX128166) dilution: 1:500.


Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG14 antibody used in Western Blot (WB). GTX128166

GTX128166 WB Image

Various whole cell and tissue extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX128166) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf