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ATG14 antibody

KO/KD
Anti-ATG14 antibody used in Western Blot (WB). GTX132468
Anti-ATG14 antibody used in Western Blot (WB). GTX132468
Anti-ATG14 antibody used in Western Blot (WB). GTX132468

Cat. No. GTX132468

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATG14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

RD, HCT-116, C6, rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886655

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 14 , ATG14L , BARKOR , KIAA0831

Background

Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.

Database

Research Area

DATA IMAGES

Anti-ATG14 antibody used in Western Blot (WB). GTX132468

GTX132468 WB Image

KO/KD

Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATG14 antibody used in Western Blot (WB). GTX132468

GTX132468 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATG14 antibody used in Western Blot (WB). GTX132468

GTX132468 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

ATG14 antibody Cat. No. GTX132468

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ATG14 antibody used in Western Blot (WB). GTX132468
Date : Anonymous submitted on 20-Apr-2023
Score :
Application Tested : WB
Sample Species : Ms, Rat
Sample : mouse brain and rat brain
Amount used : 50μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : Undiluted, 4°C, >12Hr
Figure Note : ECL reagent: GTX14698
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169