Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX132468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.