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ATG16L1 antibody

Orthogonal
Anti-ATG16L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129098
Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098
Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098
Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098

Cat. No. GTX129098

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATG16L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG16L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885894

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 16 like 1 , APG16L , ATG16A , ATG16L , IBD10 , WDR30

Cellular Localization

Cytoplasm , Preautophagosomal structure membrane; Peripheral membrane protein , Cytoplasm , Preautophagosomal structure membrane

Background

Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATG16L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129098

GTX129098 ICC/IF Image

Orthogonal

ATG16L1 antibody detects ATG16L1 protein at autophagosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATG16L1 protein stained by ATG16L1 antibody (GTX129098) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098

GTX129098 IHC-P Image

ATG16L1 antibody detects ATG16L1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ATG16L1 antibody (GTX129098) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098

GTX129098 IHC-P Image

ATG16L1 antibody detects ATG16L1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
ATG16L1 antibody (GTX129098) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG16L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129098

GTX129098 IHC-P Image

ATG16L1 antibody detects ATG16L1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ATG16L1 antibody (GTX129098) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Neto da Silva K et al. J Toxicol Environ Health A 2020; 83 (4) : 153-167 Glyphosate-based herbicide impairs energy metabolism and increases autophagy in C6 astroglioma cell line.
Application : ICC/IF
Reactivity : Rat
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169