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ATG3 antibody [AT15F6]

Anti-ATG3 antibody [AT15F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57724
Anti-ATG3 antibody [AT15F6] used in Western Blot (WB). GTX57724
Anti-ATG3 antibody [AT15F6] used in Flow cytometry (FACS). GTX57724
Anti-ATG3 antibody [AT15F6] used in Western Blot (WB). GTX57724

Cat. No. GTX57724

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT15F6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all ATG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT15F6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATG5 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 3 , APG3 , APG3L , APG3LIKE , ATG3 , PC396

Cellular Localization

Cytoplasm

Background

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-ATG3 antibody [AT15F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57724

GTX57724 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57724 ATG3 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-ATG3 antibody [AT15F6] used in Western Blot (WB). GTX57724

GTX57724 WB Image

WB analysis of various samples using GTX57724 ATG3 antibody.
Lane 1 : K562 whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : Jurkat whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-ATG3 antibody [AT15F6] used in Flow cytometry (FACS). GTX57724

GTX57724 FACS Image

FACS analysis of HeLa cells using GTX57724 ATG3 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

Anti-ATG3 antibody [AT15F6] used in Western Blot (WB). GTX57724

GTX57724 WB Image

WB analysis of various samples using GTX57724 ATG3 antibody.
Lane 1 : 293T cell lysate
Lane 2 : ATG3 transfected 293T cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399