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ATG3 antibody

Anti-ATG3 antibody used in Western Blot (WB). GTX128065
Anti-ATG3 antibody used in Western Blot (WB). GTX128065
Anti-ATG3 antibody used in Western Blot (WB). GTX128065
Anti-ATG3 antibody used in Western Blot (WB). GTX128065
Anti-ATG3 antibody used in Western Blot (WB). GTX128065

Cat No. GTX128065

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa , K562 , THP-1 , HL-60 , Jurkat , Raji , C8D30 , NIH-3T3 , BCL-1 , Raw264.7

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 3 , APG3 , APG3-LIKE , APG3L , PC3-96

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ATG3 antibody used in Western Blot (WB). GTX128065

GTX128065 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody (GTX128065) diluted at 1:1000.

Anti-ATG3 antibody used in Western Blot (WB). GTX128065

GTX128065 WB Image

ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:2000

Anti-ATG3 antibody used in Western Blot (WB). GTX128065

GTX128065 WB Image

ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
C. 30 μg HL-60 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000

Anti-ATG3 antibody used in Western Blot (WB). GTX128065

GTX128065 WB Image

ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000

Anti-ATG3 antibody used in Western Blot (WB). GTX128065

GTX128065 WB Image

ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000

REFERENCE

There are currently no references for ATG3 antibody (GTX128065). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169