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ATG3 antibody

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30073
Anti-ATG3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30073
Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30073
Anti-ATG3 antibody used in Western Blot (WB). GTX30073

Cat. No. GTX30073

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ATG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 3 , APG3 , APG3-LIKE , APG3L , PC3-96

Cellular Localization

Cytoplasm

Background

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30073

GTX30073 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX30073 ATG3 antibody.
Dilution : 1:200

Anti-ATG3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30073

GTX30073 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX30073 ATG3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX30073

GTX30073 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX30073 ATG3 antibody.
Dilution : 1:200

Anti-ATG3 antibody used in Western Blot (WB). GTX30073

GTX30073 WB Image

WB analysis of various sample lysates using GTX30073 ATG3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399