GeneTex

  • Country / Location Selection

United States (US)

ATG3 antibody

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85005
Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85005
Anti-ATG3 antibody used in Western Blot (WB). GTX85005

Cat. No. GTX85005

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ATG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse Kidney Tissue Lysate(GTX27936) , Human Kidney Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3.The immunogen is located within amino acids 180 - 230 of ATG3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 3 , APG3 , APG3-LIKE , APG3L , PC3-96

Cellular Localization

Cytoplasm

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

Database

Research Area

DATA IMAGES

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85005

GTX85005 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85005 ATG3 antibody.
Working concentration : 20 μg/ml

Anti-ATG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85005

GTX85005 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85005 ATG3 antibody.
Working concentration : 5 μg/ml

Anti-ATG3 antibody used in Western Blot (WB). GTX85005

GTX85005 WB Image

WB analysis of mouse kidney tissue lysate using GTX85005 ATG3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

There are currently no references for ATG3 antibody (GTX85005). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ATG3 antibody (GTX85005). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399