GeneTex
United States (US)

ATG4A antibody

GTX31941_3089_WB_20180221_18121410_496.jpg
GTX31941_1625_IHC-P_20180221_18121410_183.jpg

Cat No. GTX31941

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

EL4 Cell Lysate(GTX27183) , Human Spleen Tissue Slide

Specificity/Sensitivity

At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A.The immunogen is located within the last 50 amino acids of ATG4A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Autophagy Related 4A Cysteine Peptidase,Apg4A,Autl2,Atg4A

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31941_3089_WB_20180221_18121410_496.jpg

GTX31941 WB Image

WB analysis of EL4 cell lysate using GTX31941 ATG4A antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX31941_1625_IHC-P_20180221_18121410_183.jpg

GTX31941 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31941 ATG4A antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329