GeneTex
United States (US)

ATG4B antibody

GTX31942_1627_IHC-P_20180221_18121410_925.jpg
GTX31942_1626_IHC-P_20180221_18121410_298.jpg
GTX31942_3090_WB_20180221_18121410_334.jpg

Cat No. GTX31942

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Ramos Cell Lysate(GTX23955) , Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide

Specificity/Sensitivity

At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.The immunogen is located within the last 50 amino acids of ATG4B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

autophagy related 4B, cysteine peptidase Antibody , APG4B Antibody , AUTL1 Antibody

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX31942_1627_IHC-P_20180221_18121410_925.jpg

GTX31942 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31942 ATG4B antibody.
Working concentration : 20 μg/ml

GTX31942_1626_IHC-P_20180221_18121410_298.jpg

GTX31942 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31942 ATG4B antibody.
Working concentration : 5 μg/ml

GTX31942_3090_WB_20180221_18121410_334.jpg

GTX31942 WB Image

WB analysis of (A) Ramos and (B) Jurkat cell lysate using GTX31942 ATG4B antibody.
Working concentration : 1 μg/ml