APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Ramos Cell Lysate(GTX23955) , Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide
Product Note
At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.The immunogen is located within the last 50 amino acids of ATG4B.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
autophagy related 4B cysteine peptidase , APG4B , AUTL1
Cellular Localization
Cytoplasm
Background
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31942 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31942 ATG4B antibody.
Working concentration : 5 μg/ml
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GTX31942 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31942 ATG4B antibody.
Working concentration : 20 μg/ml
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GTX31942 WB Image
WB analysis of (A) Ramos and (B) Jurkat cell lysate using GTX31942 ATG4B antibody.
Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for ATG4B antibody (GTX31942). Be the first to share your publications with this product.
REVIEW
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