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ATG4B antibody

Anti-ATG4B antibody used in IHC (Paraffin sections) (IHC-P). GTX85759
Anti-ATG4B antibody used in Western Blot (WB). GTX85759

Cat. No. GTX85759

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Chicken, Pufferfish, Xenopus
Package
50 μg ($539)
See all ATG4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
IHC-P 10 μg/ml
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% Gelatin

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A portion of amino acid350-400of human APG4B.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 4B cysteine peptidase , APG4B , ATG4B , AUTL1

Cellular Localization

Cytoplasm

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ATG4B antibody used in IHC (Paraffin sections) (IHC-P). GTX85759

GTX85759 IHC-P Image

IHC-P analysis of human heart tissue using GTX85759 ATG4B antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 10 μg/ml

Anti-ATG4B antibody used in Western Blot (WB). GTX85759

GTX85759 WB Image

WB analysis of HeLa cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using GTX85759 ATG4B antibody.
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539