GeneTex
United States (US)

ATG4B antibody

ATG4B-antibody-GTX87767-IHC-1_18122102_220.jpg
ATG4B-antibody-GTX87767-WB-1_18122102_693.jpg

Cat No. GTX87767

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

ATG4B Antibody detects endogenous levels of total ATG4B protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ATG4B.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATG4B, APG4 autophagy4 homolog B, EC3.4.22., APG4 autophagy 4 homolog B, EC 3.4.22., EC 3.4.22.-

Background

Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
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Database

Research Area

DATA IMAGES
ATG4B-antibody-GTX87767-IHC-1_18122102_220.jpg

GTX87767 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide.

ATG4B-antibody-GTX87767-WB-1_18122102_693.jpg

GTX87767 WB Image

Western blot analysis of extracts from Jurkat cells, using ATG4B Antibody. The lane on the right is treated with the synthesized peptide.