*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
ATG4B Antibody detects endogenous levels of total ATG4B protein.
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antiserum was produced against synthesized peptide derived from human ATG4B.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ATG4B, APG4 autophagy4 homolog B, EC3.4.22., APG4 autophagy 4 homolog B, EC 3.4.22., EC 3.4.22.-
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
GTX87767 IHC-P Image
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using ATG4B Antibody. The picture on the right is treated with the synthesized peptide.
GTX87767 WB Image
Western blot analysis of extracts from Jurkat cells, using ATG4B Antibody. The lane on the right is treated with the synthesized peptide.