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ATG4C antibody

Anti-ATG4C antibody used in Western Blot (WB). GTX129488
Anti-ATG4C antibody used in Immunoprecipitation (IP). GTX129488
Anti-ATG4C antibody used in Western Blot (WB). GTX129488

Cat No. GTX129488

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG4C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 4C cysteine peptidase , APG4-C , APG4C , AUTL1 , AUTL3

Cellular Localization

Cytoplasm

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATG4C antibody used in Western Blot (WB). GTX129488

GTX129488 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4C antibody (GTX129488) diluted at 1:1000.

Anti-ATG4C antibody used in Immunoprecipitation (IP). GTX129488

GTX129488 IP Image

Immunoprecipitation of ATG4C protein from 293T whole cell extracts using 5 μg of ATG4C antibody (GTX129488).
Western blot analysis was performed using ATG4C antibody (GTX129488).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATG4C antibody used in Western Blot (WB). GTX129488

GTX129488 WB Image

ATG4C antibody detects ATG4C protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
ATG4C antibody (GTX129488) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf