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ATG4D antibody [N3C3]

Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114
Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114
Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114
Anti-ATG4D antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106114

Cat. No. GTX106114

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATG4D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036290

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 4D cysteine peptidase , APG4-D , APG4D , AUTL4

Cellular Localization

Cytoplasm

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114

GTX106114 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using ATG4D(GTX106114) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114

GTX106114 IHC-P Image

ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
ATG4D antibody [N3C3] (GTX106114) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG4D antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX106114

GTX106114 IHC-P Image

ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
ATG4D antibody [N3C3] (GTX106114) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG4D antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106114

GTX106114 ICC/IF Image

ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG4D protein stained by ATG4D antibody [N3C3] (GTX106114) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169