GeneTex
United States (US)

ATG5 antibody [N1C3]

GTX102361_39728_WB_M_Brain_19011506_615.jpg
GTX102361_39728_20160413_IHC-P_H_19011506_681.jpg

Cat No. GTX102361

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, autophagy protein 5 antibody, apoptosis-specific protein antibody, apoptosis specific protein antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES
GTX102361_39728_WB_M_Brain_19011506_615.jpg

GTX102361 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102361 diluted at 1:500

GTX102361_39728_20160413_IHC-P_H_19011506_681.jpg

GTX102361 IHC-P Image

ATG5 antibody [N1C3] detects ATG5 protein at cytoplasm in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ATG5 antibody [N1C3] (GTX102361) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf