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ATG5 antibody

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309

Cat No. GTX113309

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, PLA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , HeLa , HeLa (chloroquine treatment for 24hr)

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633458

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309

GTX113309 IHC-P Image

ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ATG5 antibody (GTX113309) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: PC-3
12% SDS PAGE
GTX113309 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ATG5 antibody Cat No. GTX113309

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (DMEM and CM) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf