ATG5 antibody detects autophagy related 5 (ATG5), a ~32kDa key protein that contributes to the formation of autophagic vesicles when conjugated to ATG12 through the actions of ATG7 and ATG10. It plays key roles in mitochondrial function, lymphocyte biology, and axonal maintenance, among others. Dysregulation of ATG5 is associated with several diseases, including sporadic Parkinson's disease, childhood asthma, and cancers.
*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
NT2D1 , PC-3 , HeLa , HeLa (chloroquine treatment for 24hr)
Bovine, Chicken, Xenopus laevis(>80% identity)
0.1M Tris-Glycine (pH7), 10% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.