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ATG5 antibody

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309
Anti-ATG5 antibody used in Western Blot (WB). GTX113309

Cat No. GTX113309

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, PLA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

ATG5 antibody detects autophagy related 5 (ATG5), a ~32kDa key protein that contributes to the formation of autophagic vesicles when conjugated to ATG12 through the actions of ATG7 and ATG10. It plays key roles in mitochondrial function, lymphocyte biology, and axonal maintenance, among others. Dysregulation of ATG5 is associated with several diseases, including sporadic Parkinson's disease, childhood asthma, and cancers.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , HeLa , HeLa (chloroquine treatment for 24hr)

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633458

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113309

GTX113309 IHC-P Image

ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ATG5 antibody (GTX113309) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: PC-3
12% SDS PAGE
GTX113309 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113309

GTX113309 ICC/IF Image

ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min.
Green: ATG5 protein stained by ATG5 antibody (GTX113309) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ATG5 antibody used in Western Blot (WB). GTX113309

GTX113309 WB Image

ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ATG5 antibody Cat No. GTX113309

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (DMEM and CM) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GTX113309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf