GeneTex
United States (US)

ATG5 antibody

GTX31372_967_IHC-P_20180221_18121410_623.jpg
GTX31372_2572_WB_20180221_18121410_911.jpg

Cat No. GTX31372

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Mouse Spleen Tissue Lysate(GTX27937) , Human Spleen Tissue Slide

Specificity/Sensitivity

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.The immunogen is located within the first 50 amino acids of ATG5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX31372_967_IHC-P_20180221_18121410_623.jpg

GTX31372 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31372 ATG5 antibody.
Working concentration : 2.5 μg/ml

GTX31372_2572_WB_20180221_18121410_911.jpg

GTX31372 WB Image

WB analysis of mouse spleen tissue lysate using GTX31372 ATG5 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 339