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ATG5 antibody

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31770
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31770
Anti-ATG5 antibody used in Western Blot (WB). GTX31770
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31770

Cat No. GTX31770

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Rat Spleen Tissue Lysate(GTX27945) , Human Spleen Tissue Slide

Specificity/Sensitivity

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.The immunogen is located within the first 50 amino acids of ATG5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31770

GTX31770 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31770 ATG5 antibody.
Working concentration : 2.5 μg/ml

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX31770

GTX31770 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31770 ATG5 antibody.
Working concentration : 20 μg/ml

Anti-ATG5 antibody used in Western Blot (WB). GTX31770

GTX31770 WB Image

WB analysis of rat spleen tissue lysate using GTX31770 ATG5 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31770

GTX31770 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31770 ATG5 antibody.
Dilution : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339