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ATG5 antibody

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48583
Anti-ATG5 antibody used in Western Blot (WB). GTX48583
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583
Anti-ATG5 antibody used in Western Blot (WB). GTX48583

Cat No. GTX48583

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, PLA

Reactivity

Human, Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus, Primate
Package
50 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:250
IHC-P 1:400
FACS Assay dependent
IP 1:10 - 1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10620228

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48583

GTX48583 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48583 ATG5 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-ATG5 antibody used in Western Blot (WB). GTX48583

GTX48583 WB Image

WB analysis of HeLa cell lysate using GTX48583 ATG5 antibody.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583

GTX48583 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX48583 ATG5 antibody.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583

GTX48583 IHC-P Image

IHC-P analysis of human liver tissue using GTX48583 ATG5 antibody.
Dilution : 2.5 μg/ml

Anti-ATG5 antibody used in Western Blot (WB). GTX48583

GTX48583 WB Image

WB analysis of HeLa, A431, and MEF cell lysate using GTX48583 ATG5 antibody.
Dilution : 2 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf