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ATG5 antibody

Anti-ATG5 antibody used in Western Blot (WB). GTX48583
Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48583
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583
Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583
Anti-ATG5 antibody used in Western Blot (WB). GTX48583

Cat. No. GTX48583

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, PLA

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus, Primate
Package
50 μl ($399)
See all ATG5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:250
IHC-P 1:400
FACS Assay dependent
IP 1:10 - 1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10620228

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-ATG5 antibody used in Western Blot (WB). GTX48583

GTX48583 WB Image

WB analysis of HeLa cell lysate using GTX48583 ATG5 antibody.

Anti-ATG5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48583

GTX48583 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48583 ATG5 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583

GTX48583 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX48583 ATG5 antibody.

Anti-ATG5 antibody used in IHC (Paraffin sections) (IHC-P). GTX48583

GTX48583 IHC-P Image

IHC-P analysis of human liver tissue using GTX48583 ATG5 antibody.
Dilution : 2.5 μg/ml

Anti-ATG5 antibody used in Western Blot (WB). GTX48583

GTX48583 WB Image

WB analysis of HeLa, A431, and MEF cell lysate using GTX48583 ATG5 antibody.
Dilution : 2 μg/ml

REFERENCE

Application Reference

Lenain C et al. Carcinogenesis 2015; 36 (11) : 1263-74 Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399