GeneTex

  • Country / Location Selection

United States (US)

ATG7 antibody [GT1218]

Recombinant antibody
Anti-ATG7 antibody [GT1218] used in Western Blot (WB). GTX01526
Anti-ATG7 antibody [GT1218] used in Western Blot (WB). GTX01526
Anti-ATG7 antibody [GT1218] used in IHC (Paraffin sections) (IHC-P). GTX01526
Anti-ATG7 antibody [GT1218] used in IHC (Paraffin sections) (IHC-P). GTX01526

Cat. No. GTX01526

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1218

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Atg7(Apg7).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APG7L,APG7LIKE,ATG7,GSA7,autophagy related 7,APG7

Cellular Localization

Cytoplasm,Preautophagosomal structure

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-ATG7 antibody [GT1218] used in Western Blot (WB). GTX01526

GTX01526 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG7 antibody [GT1218] (GTX01526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG7 antibody [GT1218] used in Western Blot (WB). GTX01526

GTX01526 WB Image

WB analysis of various samples using GTX01526 ATG7 antibody [GT1218].
Dilution : 1:1000
Loading : 25 μg

Anti-ATG7 antibody [GT1218] used in IHC (Paraffin sections) (IHC-P). GTX01526

GTX01526 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX01526 ATG7 antibody [GT1218].
Dilution : 1:100

Anti-ATG7 antibody [GT1218] used in IHC (Paraffin sections) (IHC-P). GTX01526

GTX01526 IHC-P Image

IHC-P analysis of human esophageal cancer tissue section using GTX01526 ATG7 antibody [GT1218].
Dilution : 1:100

REFERENCE

Application Reference

Hsu CY et al. Microbiol Spectr 2021; 9 (3) : e0151921 A Novel Dibenzoxazepine Attenuates Intracellular Salmonella Typhimurium Oxidative Stress Resistance.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for ATG7 antibody [GT1218] (GTX01526). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399