GeneTex
United States (US)

ATG7 antibody

GTX16984_30_ICC-IF_20180221_18121410_288.jpg
GTX16984_29_ICC-IF_20180221_18121410_628.jpg
GTX16984_2306_WB_20180221_18121410_822.jpg

Cat No. GTX16984

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Caco-2 Cell Lysate(GTX23950) , MCF7 Cell Slide(GTX25536) , MCF7 Cell Lysate

Specificity/Sensitivity

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7.The immunogen is located within amino acids 590 - 640 of APG7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Autophagy Related 7,Apg7-Like,Apg7L,Gsa7,Atg7

Background

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES
GTX16984_30_ICC-IF_20180221_18121410_288.jpg

GTX16984 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX16984 ATG7 antibody.
Working concentration : 20 μg/ml

GTX16984_29_ICC-IF_20180221_18121410_628.jpg

GTX16984 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX16984 ATG7 antibody.
Working concentration : 10 μg/ml

GTX16984_2306_WB_20180221_18121410_822.jpg

GTX16984 WB Image

WB analysis of Caco-2 cell lysate using GTX16984 ATG7 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Package List Price ($)
$ 339