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ATG7 antibody

Anti-ATG7 antibody used in Western Blot (WB). GTX16985
Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16985
Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16985

Cat. No. GTX16985

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ATG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 4 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

L1210 Cell Slide(GTX25545) , L1210 Cell Lysate(GTX27184)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APG7 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human APG7.The immunogen is located within the first 50 amino acids of APG7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 7 , APG7-LIKE , APG7L , GSA7

Cellular Localization

Cytoplasm

Background

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-ATG7 antibody used in Western Blot (WB). GTX16985

GTX16985 WB Image

WB analysis of L1210 cell lysate using GTX16985 ATG7 antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml

Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16985

GTX16985 ICC/IF Image

ICC/IF analysis of L1210 cells using GTX16985 ATG7 antibody.
Working concentration : 10 μg/ml

Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16985

GTX16985 ICC/IF Image

ICC/IF analysis of L1210 cells using GTX16985 ATG7 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399