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ATG7 antibody

Anti-ATG7 antibody used in Western Blot (WB). GTX32459
Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32459
Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459
Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459

Cat. No. GTX32459

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887657

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 7 , APG7-LIKE , APG7L , GSA7

Cellular Localization

Cytoplasm

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-ATG7 antibody used in Western Blot (WB). GTX32459

GTX32459 WB Image

WB analysis of various sample lysates using GTX32459 ATG7 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32459

GTX32459 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32459 ATG7 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459

GTX32459 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX32459 ATG7 antibody.
Dilution : 1:100

Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459

GTX32459 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32459 ATG7 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Samaka RM et al. Clin Cosmet Investig Dermatol 2020; 13 : 49-58 Does the Autophagy Related Gene 7 (ATG7) Have a Role in Non-Melanoma Skin Cancer?
Application : IHC-P
Reactivity : Human
Aoki A et al. Am J Pathol 2018; (Epub) Trophoblast-Specific Conditional Atg7 Knockout Mice Develop Gestational Hypertension.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399