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ATG7 antibody

Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32459
Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459
Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459
Anti-ATG7 antibody used in Western Blot (WB). GTX32459

Cat No. GTX32459

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 7 , APG7-LIKE , APG7L , GSA7

Cellular Localization

Cytoplasm

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-ATG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32459

GTX32459 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32459 ATG7 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459

GTX32459 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32459 ATG7 antibody.
Dilution : 1:100

Anti-ATG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX32459

GTX32459 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX32459 ATG7 antibody.
Dilution : 1:100

Anti-ATG7 antibody used in Western Blot (WB). GTX32459

GTX32459 WB Image

WB analysis of various sample lysates using GTX32459 ATG7 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329