ATG9A antibody

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 100% MeOH for 5 min.
Green: ATG9A protein stained by ATG9A antibody (GTX128427) diluted at 1:500.
Blue: Hoechst 33342 staining.

ATG9A antibody detects ATG9A protein on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATG9A antibody (GTX128427) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ATG9A antibody detects ATG9A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG9A antibody immunoprecipitates ATG9A protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427)
7.5 % SDS-PAGE
The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATG9A stained by ATG9A antibody (GTX128427) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal PLoS One in 2017. PMID: 28910396

The data was published in the journal Nat Commun in 2016. PMID: 27510922