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ATG9A antibody

Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Immunoprecipitation (IP). GTX128427
Anti-ATG9A antibody used in IHC (Paraffin sections) (IHC-P). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

Cat No. GTX128427

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

293T , A431 , HeLa , RD

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy related 9A , APG9L1 , MGD3208 , mATG9

Cellular Localization

Cytoplasmic vesicle , autophagosome membrane

Background

Plays a role in autophagy.

Database

Research Area

DATA IMAGES

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

GTX128427 ICC/IF Image

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 100% MeOH for 5 min.
Green: ATG9A protein stained by ATG9A antibody (GTX128427) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Immunoprecipitation (IP). GTX128427

GTX128427 IP Image

ATG9A antibody immunoprecipitates ATG9A protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427)
7.5 % SDS-PAGE
The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-ATG9A antibody used in IHC (Paraffin sections) (IHC-P). GTX128427

GTX128427 IHC-P Image

ATG9A antibody detects ATG9A protein on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATG9A antibody (GTX128427) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

GTX128427 ICC/IF Image

The ICC/IF analysis of ATG9A antibody was published by Karanasios E and colleagues in the journal Nat Commun in 2016.PMID: 27510922

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169