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ATG9B antibody

Orthogonal
Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132439
Anti-ATG9B antibody used in Western Blot (WB). GTX132439

Cat. No. GTX132439

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATG9B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886648

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 9B , APG9L2 , NOS3AS , SONE

Cellular Localization

Cytoplasmic vesicle , autophagosome membrane

Background

Plays a role in autophagy.

Database

Research Area

DATA IMAGES

Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132439

GTX132439 ICC/IF Image

Orthogonal

ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500 μM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG9B protein stained by ATG9B antibody (GTX132439) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ATG9B antibody used in Western Blot (WB). GTX132439

GTX132439 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody (GTX132439) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169