Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody (GTX132439) diluted at 1:1000.

ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500 μM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG9B protein stained by ATG9B antibody (GTX132439) diluted at 1:500.
Blue: Hoechst 33342 staining.