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ATG9B antibody

Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85184
Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85184
Anti-ATG9B antibody used in Western Blot (WB). GTX85184

Cat. No. GTX85184

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ATG9B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B.The immunogen is located within amino acids 850 - 900 of ATG9B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 9B , APG9L2 , NOS3AS , SONE

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it’s highly expressed in placenta and pituitary gland.

Database

Research Area

DATA IMAGES

Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85184

GTX85184 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85184 ATG9B antibody.
Working concentration : 10 μg/ml

Anti-ATG9B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85184

GTX85184 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85184 ATG9B antibody.
Working concentration : 20 μg/ml

Anti-ATG9B antibody used in Western Blot (WB). GTX85184

GTX85184 WB Image

WB analysis of HeLa cell lysate using GTX85184 ATG9B antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399