GeneTex
  • Country / Location Selection

United States (US)

ATGL antibody [N1C1]

Anti-ATGL antibody [N1C1] used in Western Blot (WB). GTX109941
Anti-ATGL antibody [N1C1] used in Western Blot (WB). GTX109941

Cat No. GTX109941

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , mouse white adipose

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATGL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951306

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

patatin like phospholipase domain containing 2 , 1110001C14Rik , ATGL , FP17548 , PEDF-R , TTS-2.2 , TTS2 , iPLA2zeta

Background

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATGL antibody [N1C1] used in Western Blot (WB). GTX109941

GTX109941 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATGL antibody [N1C1] (GTX109941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATGL antibody [N1C1] used in Western Blot (WB). GTX109941

GTX109941 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATGL antibody [N1C1] (GTX109941) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ATGL antibody [N1C1] Cat No. GTX109941

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Epidydimal white adipocytes tissue lysate
Amount used : 40 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Figure Note : NCD: Normal chow diet
HFD: High fat diet
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169