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ATIC antibody

Anti-ATIC antibody used in Western Blot (WB). GTX103036
Anti-ATIC antibody used in IHC (Paraffin sections) (IHC-P). GTX103036
Anti-ATIC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103036

Cat. No. GTX103036

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATIC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATIC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase , AICAR , AICARFT , HEL-S-70p , IMPCHASE , PURH

Background

This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATIC antibody used in Western Blot (WB). GTX103036

GTX103036 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
ATIC antibody
GTX103036 diluted at 1:1000

Anti-ATIC antibody used in IHC (Paraffin sections) (IHC-P). GTX103036

GTX103036 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATIC(GTX103036) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATIC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103036

GTX103036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATIC(GTX103036) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169