GeneTex
United States (US)

ATIC antibody

GTX103036_WB_19011506_516.jpg
GTX103036_39848_IHC_19011506_737.jpg
GTX103036_IFA_19011506_138.jpg

Cat No. GTX103036

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATIC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AICAR antibody, AICARFT antibody, FLJ93545 antibody, IMPCHASE antibody, PURH antibody, ATIC antibody, bifunctional purine biosynthesis protein PURH antibody, AICARFT/IMPCHASE antibody, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase antibody, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody

Background

This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq]

Database

DATA IMAGES
GTX103036_WB_19011506_516.jpg

GTX103036 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
ATIC antibody
GTX103036 diluted at 1:1000

GTX103036_39848_IHC_19011506_737.jpg

GTX103036 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATIC(GTX103036) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103036_IFA_19011506_138.jpg

GTX103036 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATIC(GTX103036) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169