Application Note
Recommended Starting Dilutions:
For WB: Use at 1:100.
For IP: Use at an assay dependent dilution.
For IHC: Use at 1:50. Analysis was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For ICC/IF: Use at 1:500.
Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
Raji and lymphoblastoid nuclear lysate
Form
Liquid
Buffer
TCS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Purification
Unpurified
From hybridoma culture supernatant
Conjugation
Unconjugated
RRID
AB_377546
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1
Cellular Localization
Nucleus , Cytoplasmic vesicle
Background
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
Database
Research Area