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ATM antibody [2C1] (supernatant)

Anti-ATM antibody [2C1] (supernatant) used in IHC (Paraffin sections) (IHC-P). GTX77613
Anti-ATM antibody [2C1] (supernatant) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77613
Anti-ATM antibody [2C1] (supernatant) used in Western Blot (WB). GTX77613

Cat No. GTX77613

Host

Mouse

Clonality

Monoclonal

Clone Name

2C1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
1000 μl ($379)

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at 1:100.
For IP: Use at an assay dependent dilution.
For IHC: Use at 1:50. Analysis was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For ICC/IF: Use at 1:500.
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

351 kDa. ( Note )

Positive Control

Raji and lymphoblastoid nuclear lysate

PROPERTIES

Form

Liquid

Buffer

TCS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Purification

Unpurified
From hybridoma culture supernatant

Conjugation

Unconjugated

RRID

AB_377546

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [2C1] (supernatant) used in IHC (Paraffin sections) (IHC-P). GTX77613

GTX77613 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATM(GTX77613) antibody at 1:50 dilution.

Anti-ATM antibody [2C1] (supernatant) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77613

GTX77613 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM(GTX77613) antibody at 1:100 dilution.

Anti-ATM antibody [2C1] (supernatant) used in Western Blot (WB). GTX77613

GTX77613 WB Image

Sample (30 ug of whole cell lysate)

A: 293T

B: A431

C: HeLa

D: HepG2

5% SDS PAGE

GTX77613 diluted at 1:100

REFERENCE

REVIEW

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Package List Price ($)
$ 379