Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.
SK-N-SH , HeLa , HeLa nuclear extract
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.