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ATM antibody [2C1]

KO/KD Orthogonal
GTX70103_42520_20161124_WB_shRNA_watermark_18121411_436.jpg
ATM-antibody-2C1-GTX70103-IHC-p-2_18121409_181.jpg
ATM-antibody-2C1-GTX70103-IP-1_18121409_950.jpg
ATM-antibody-2C1-GTX70103-IHC-p-1_18121409_111.jpg
GTX70103_42541_20160721_WB_18121411_814.jpg
GTX70103_42821_20160420_WB_Fraction_19021719_872.jpg

Cat No. GTX70103

Host Mouse
Clonality Monoclonal
Clone Name 2C1
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

370 kDa. ( Note )

Positive Control

SK-N-SH , HeLa , HeLa nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Background

The ATM antibody, clone 2C1, recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Database

Research Area

DATA IMAGES
GTX70103_42520_20161124_WB_shRNA_watermark_18121411_436.jpg

GTX70103 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

ATM-antibody-2C1-GTX70103-IHC-p-2_18121409_181.jpg

GTX70103 IHC-P Image

Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

ATM-antibody-2C1-GTX70103-IP-1_18121409_950.jpg

GTX70103 WB Image

Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (GTX70103) by western blot or immunoprecipitation.

ATM-antibody-2C1-GTX70103-IHC-p-1_18121409_111.jpg

GTX70103 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

GTX70103_42541_20160721_WB_18121411_814.jpg

GTX70103 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:1000.

GTX70103_42821_20160420_WB_Fraction_19021719_872.jpg

GTX70103 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf