GeneTex
  • Country / Location Selection

United States (US)

ATM antibody [2C1]

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

Cat No. GTX70103

Host Mouse
Clonality Monoclonal
Clone Name 2C1
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Monkey
Package
100 μl ($319)
APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

370 kDa. ( Note )

Positive Control

SK-N-SH , HeLa , HeLa nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atm Serine/Threonine Kinase,At1,Ata,Atc,Atd,Atdc,Ate,Tel1,Telo1,Atm

Cellular Localization

Nucleus

Background

The ATM antibody, clone 2C1, recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Database

Research Area

DATA IMAGES
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (GTX70103) by western blot or immunoprecipitation.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:1000.

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

HeLa whole cell extract and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

ATM antibody [2C1] detects ATM protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATM stained by ATM antibody [2C1] (GTX70103) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
There are currently no reviews for ATM antibody [2C1] (GTX70103). Be the first to share your experience with this product.
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319