GeneTex
  • Country / Location Selection

United States (US)

ATM antibody [3E8]

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

Cat No. GTX70105

Host

Mouse

Clonality

Monoclonal

Clone Name

3E8

Isotype

IgG1

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
IHC-P Assay dependent
IP 2-10 μg/ml
Not tested in other applications.

Calculated MW

370 kDa. ( Note )

Positive Control

Raji, HeLa, or Akata

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Atm Serine/Threonine Kinase , At1 , Ata , Atc , Atd , Atdc , Ate , Tel1 , Telo1 , Atm

Cellular Localization

Nuclear

Background

ATM-3E8 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Database

Research Area

DATA IMAGES

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 μg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105

GTX70105 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
5% SDS PAGE
GTX70105 diluted at 1:1000

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

REFERENCE

REVIEW

There are currently no reviews for ATM antibody [3E8] (GTX70105). Be the first to share your experience with this product.