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ATM antibody [3E8]

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

Cat No. GTX70105

Host

Mouse

Clonality

Monoclonal

Clone Name

3E8

Isotype

IgG1

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
IHC-P Assay dependent
IP 2-10 μg/ml
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

Raji, HeLa, or Akata

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368163

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 μg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105

GTX70105 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [3E8] (GTX70105) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

REFERENCE

REVIEW

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Package List Price ($)
$ 379
Custom Conjugations