GeneTex

  • Country / Location Selection

United States (US)

ATM antibody [3E8]

Orthogonal
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

Cat. No. GTX70105

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E8

Isotype

 IgG1

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IHC-P Assay dependent
IP 2-10 μg/ml
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

Raji, HeLa, or Akata

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 μg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105

GTX70105 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [3E8] (GTX70105) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

REFERENCE

Application Reference

Fini L et al. Carcinogenesis 2008; 29 (1) : 139-46 Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines.
Application : WB
Reactivity : Human
Bai L et al. Gastroenterology 2006; 131 (3) : 841-52 Recruitment of ataxia-telangiectasia mutated to the p21(waf1) promoter by ZBP-89 plays a role in mucosal protection.
Shiotani B et al. Mol Cancer Res 2006; 4 (2) : 125-33 Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1.
Application : WB
Reactivity : Human

Clone Reference

Post S et al. Proc Natl Acad Sci U S A 2001; 98 (23):13102-7 Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage.
Chen MJ et al. J Biol Chem 2001; 276 (19):16580-6 ATM-dependent phosphorylation of human Rad9 is required for ionizing radiation-induced checkpoint activation.
Submit a Reference

REVIEW

There are currently no reviews for ATM antibody [3E8] (GTX70105). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
Custom Conjugations