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ATM antibody [5C2]

KO/KD Orthogonal
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

Cat. No. GTX70107

Reference 24
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C2

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

Raji , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

Detection of human ATM protein using GeneTex ATM 5C2 monoclonal antibody (GTX70107) in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2). Also available 200ug size GTX70108.

The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107

GTX70107 ICC/IF Image

ATM antibody [5C2] detects ATM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM protein stained by ATM antibody [5C2] (GTX70107) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [5C2] (GTX70107) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yeo AJ et al. Mol Metab 2021; 54 : 101354 An anaplerotic approach to correct the mitochondrial dysfunction in ataxia-telangiectasia (A-T).
Application : WB, ICC/IF
Reactivity : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6) : 1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
Application : ICC/IF
Reactivity : Human
Yeo AJ et al. Am J Respir Crit Care Med 2017; Loss of ATM in Airway Epithelial Cells is Associated with Susceptibility to Oxidative Stress.
Du F et al. Biochem Biophys Res Commun 2014; 452 (4) : 1034-9 Dimer monomer transition and dimer re-formation play important role for ATM cellular function during DNA repair.
Application : WB
Reactivity : Human
Rankin AM et al. J Cell Physiol 2012; 227 (6) : 2586-94 Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells.
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Submit a Reference

REVIEW

ATM antibody [5C2] Cat. No. GTX70107

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010. PMID: 20663147
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010. PMID: 20663147
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010. PMID: 20663147
Submit a Review
Application Reference
Yeo AJ et al. Mol Metab 2021; 54:101354 An anaplerotic approach to correct the mitochondrial dysfunction in ataxia-telangiectasia (A-T).
Application : WB, ICC/IF
Reactivity : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6):1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
Application : ICC/IF
Reactivity : Human
Yeo AJ et al. Am J Respir Crit Care Med 2017; Loss of ATM in Airway Epithelial Cells is Associated with Susceptibility to Oxidative Stress.
Du F et al. Biochem Biophys Res Commun 2014; 452 (4):1034-9 Dimer monomer transition and dimer re-formation play important role for ATM cellular function during DNA repair.
Application : WB
Reactivity : Human
Javvadi P et al. Mol Cancer Res 2012; 10 (10):1359-68 Threonine 2609 phosphorylation of the DNA-dependent protein kinase is a critical prerequisite for epidermal growth factor receptor-mediated radiation resistance.
Application : WB
Reactivity : Human
Vogel R et al. J Virol 2012; 86 (1):143-55 Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection.
Application : WB
Rankin AM et al. J Cell Physiol 2012; 227 (6):2586-94 Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells.
Gapud EJ et al. Proc Natl Acad Sci U S A 2011; 108 (5):44743 Ataxia telangiectasia mutated (Atm) and DNA-PKcs kinases have overlapping activities during chromosomal signal joint formation.
Application : WB
Reactivity : Mouse
Cam H et al. Mol Cell 2010; 40 (4):509-20 mTORC1 signaling under hypoxic conditions is controlled by ATM-dependent phosphorylation of HIF-1α.
Application : IHC
Reactivity : Human
Craig AL et al. Mol Cancer 2010; 9:195 DeltaNp63 transcriptionally regulates ATM to control p53 Serine-15 phosphorylation.
Application : WB
Reactivity : Human
Gonzalez-Suarez I et al. EMBO J 2009; 28 (16):2414-27 Novel roles for A-type lamins in telomere biology and the DNA damage response pathway.
Application : WB
Reactivity : Mouse
Siehler SY et al. DNA Repair (Amst) 2009; 8 (2):242-52 Human MutL-complexes monitor homologous recombination independently of mismatch repair.
Application : WB
Ariumi Y et al. J Virol 2008; 82 (19):9639-46 The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication.
Application : ICC/IF
Reactivity : Human
Lai CK et al. Virology 2008; 370 (2):295-309 Hepatitis C virus NS3/4A protein interacts with ATM, impairs DNA repair and enhances sensitivity to ionizing radiation.
Application : ICC/IF
Reactivity : Human
Sun Y et al. Mol Cell Biol 2007; 27 (24):2411576 DNA damage-induced acetylation of lysine 3016 of ATM activates ATM kinase activity.
Application : ICC/IF
Reactivity : Human
Fernandes ND et al. J Biol Chem 2007; 282 (22):16577-84 Activation of the kinase activity of ATM by retinoic acid is required for CREB-dependent differentiation of neuroblastoma cells.
Application : WB
Reactivity : Human
Chen JH et al. FEBS Lett 2006; 580 (28-29):6669-73 Deep senescent human fibroblasts show diminished DNA damage foci but retain checkpoint capacity to oxidative stress.
Application : WB
Reactivity : Human
Sun Y et al. FEBS Lett 2006; 580 (18):896093 Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation.
Application : WB
Reactivity : Human
Roy K et al. Biochem Biophys Res Commun 2006; 344 (3):821-6 Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity.
Application : ICC/IF
Reactivity : Human
Jiang X et al. J Biol Chem 2006; 281 (23):15741-6 The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM.
Application : WB
Reactivity : Human
Shiotani B et al. Mol Cancer Res 2006; 4 (2):125-33 Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1.
Application : WB
Reactivity : Human
Fernandes N et al. J Biol Chem 2005; 280 (15):15158-64 DNA damage-induced association of ATM with its target proteins requires a protein interaction domain in the N terminus of ATM.
Application : WB, ICC/IF
Reactivity : Human
Sun Y et al. Proc Natl Acad Sci U S A 2005; 102 (37):13182-7 A role for the Tip60 histone acetyltransferase in the acetylation and activation of ATM.
Application : WB
Reactivity : Human
Lai CH et al. Proc Natl Acad Sci U S A 2004; 101 (44):15676-81 Correction of ATM gene function by aminoglycoside-induced read-through of premature termination codons.
Application : WB, IP
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations