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ATM antibody [5C2]

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

Cat No. GTX70107

Host Mouse
Clonality Monoclonal
Clone Name 5C2
Isotype IgG1
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse, Rat, Monkey
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

370 kDa. ( Note )

Positive Control

Raji , HeLa nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atm Serine/Threonine Kinase , At1 , Ata , Atc , Atd , Atdc , Ate , Tel1 , Telo1 , Atm

Cellular Localization

Nuclear

Background

ATM-5C2 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Database

Research Area

DATA IMAGES
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [5C2] (GTX70107) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107

GTX70107 ICC/IF Image

ATM antibody [5C2] detects ATM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM protein stained by ATM antibody [5C2] (GTX70107) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

Detection of human ATM protein using GeneTex ATM 5C2 monoclonal antibody (GTX70107) in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2). Also available 200ug size GTX70108.

The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319