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ATM antibody [5C2]

Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107
Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

Cat No. GTX70107

Host

Mouse

Clonality

Monoclonal

Clone Name

5C2

Isotype

IgG1

Application

WB, ICC/IF, IP, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Positive Control

Raji , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368165

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [5C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70107

GTX70107 ICC/IF Image

ATM antibody [5C2] detects ATM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM protein stained by ATM antibody [5C2] (GTX70107) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

Detection of human ATM protein using GeneTex ATM 5C2 monoclonal antibody (GTX70107) in Raji whole cell extract (lane 1) and T24 syncronized cell lysate (lane 2). Also available 200ug size GTX70108.

The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATM antibody [5C2] used in Western Blot (WB). GTX70107

GTX70107 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [5C2] (GTX70107) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

ATM antibody [5C2] Cat No. GTX70107

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Mol Cancer in 2010.PMID: 20663147
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Mol Cancer in 2010.PMID: 20663147
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Mol Cancer in 2010.PMID: 20663147
Application Reference
SDS
PBS.pdf