GeneTex
United States (US)

ATM antibody

GTX30104_1323_WB_18121410_631.jpg
GTX30104_307_IHC-P_18121410_556.jpg
GTX30104_1602_ICC-IF_18121410_803.jpg
GTX30104_1322_WB_18121410_781.jpg
GTX30104_94_FACS_18121410_720.jpg
GTX30104_173_ICC-IF_18121410_455.jpg

Cat No. GTX30104

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100
IHC-P 1:100
FACS Assay dependent
IP 1:10 - 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atm Serine/Threonine Kinase,At1,Ata,Atc,Atd,Atdc,Ate,Tel1,Telo1,Atm

Cellular Localization

Nucleus,Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30104_1323_WB_18121410_631.jpg

GTX30104 WB Image

WB analysis of Raji cell lysate using GTX30104 ATM antibody.

GTX30104_307_IHC-P_18121410_556.jpg

GTX30104 IHC-P Image

IHC-P analysis of human testis tissue using GTX30104 ATM antibody.

GTX30104_1602_ICC-IF_18121410_803.jpg

GTX30104 ICC/IF Image

ICC/IF analysis of HeLa and AT2SF cells using GTX30104 ATM antibody.
Dlilution : 1:500

GTX30104_1322_WB_18121410_781.jpg

GTX30104 WB Image

WB analysis of HeLa nuclear extract using GTX30104 ATM antibody.

GTX30104_94_FACS_18121410_720.jpg

GTX30104 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX30104 ATM antibody.
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX30104_173_ICC-IF_18121410_455.jpg

GTX30104 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30104 ATM antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
Fixation : 10% formalin
Permibilization : 0.05% Triton-X100

REFERENCE