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ATM antibody

Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30104
Anti-ATM antibody used in Western Blot (WB). GTX30104
Anti-ATM antibody used in Flow cytometry (FACS). GTX30104
Anti-ATM antibody used in Western Blot (WB). GTX30104
Anti-ATM antibody used in IHC (Paraffin sections) (IHC-P). GTX30104
Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30104

Cat. No. GTX30104

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human, Mouse
Package
50 μl ($419)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100
IHC-P 1:100
FACS Assay dependent
IP 1:10 - 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_368142

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus,Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30104

GTX30104 ICC/IF Image

ICC/IF analysis of HeLa and AT2SF cells using GTX30104 ATM antibody.
Dlilution : 1:500

Anti-ATM antibody used in Western Blot (WB). GTX30104

GTX30104 WB Image

WB analysis of Raji cell lysate using GTX30104 ATM antibody.

Anti-ATM antibody used in Flow cytometry (FACS). GTX30104

GTX30104 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX30104 ATM antibody.
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-ATM antibody used in Western Blot (WB). GTX30104

GTX30104 WB Image

WB analysis of HeLa nuclear extract using GTX30104 ATM antibody.

Anti-ATM antibody used in IHC (Paraffin sections) (IHC-P). GTX30104

GTX30104 IHC-P Image

IHC-P analysis of human testis tissue using GTX30104 ATM antibody.

Anti-ATM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30104

GTX30104 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30104 ATM antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
Fixation : 10% formalin
Permibilization : 0.05% Triton-X100

REFERENCE

Application Reference

Ariumi Y et al. J Virol 2005; 79 (5) : 392120 DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419