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ATM (phospho Ser1981) antibody [10H11.E12]

Orthogonal
Anti-ATM (phospho Ser1981) antibody [10H11.E12] used in IHC (Paraffin sections) (IHC-P). GTX30636
Anti-ATM (phospho Ser1981) antibody [10H11.E12] used in Western Blot (WB). GTX30636

Cat. No. GTX30636

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10H11.E12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:1000
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_625327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus,Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATM (phospho Ser1981) antibody [10H11.E12] used in IHC (Paraffin sections) (IHC-P). GTX30636

GTX30636 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX30636 ATM (phospho Ser1981) antibody [10H11.E12].

Anti-ATM (phospho Ser1981) antibody [10H11.E12] used in Western Blot (WB). GTX30636

GTX30636 WB Image

Orthogonal

WB analysis of irradiate or peroxidase treated human fibroblast lysate using GTX30636 ATM (phospho Ser1981) antibody [10H11.E12].

REFERENCE

Application Reference

YP Lee et al. Am J Cancer Res 2023; Combination treatment of cordycepin and radiation induces MA-10 mouse Leydig tumor cell death via ROS accumulation and DNA damage
Application : WB
Reactivity : Mouse
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6) : 1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409