GeneTex
United States (US)

ATM (phospho Ser1981) antibody [10H11.E12]

Orthogonal
GTX30636_329_IHC-P_18121410_573.jpg
GTX30636_1445_WB_18121410_325.jpg

Cat No. GTX30636

Host Mouse
Clonality Monoclonal
Clone Name 10H11.E12
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:1000
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

351 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atm Serine/Threonine Kinase,At1,Ata,Atc,Atd,Atdc,Ate,Tel1,Telo1,Atm

Cellular Localization

Nucleus,Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30636_329_IHC-P_18121410_573.jpg

GTX30636 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX30636 ATM (phospho Ser1981) antibody [10H11.E12].

GTX30636_1445_WB_18121410_325.jpg

GTX30636 WB Image

Orthogonal

WB analysis of irradiate or peroxidase treated human fibroblast lysate using GTX30636 ATM (phospho Ser1981) antibody [10H11.E12].