GeneTex
United States (US)

ATM (phospho Ser1981) antibody [10H11]

Orthogonal
GTX15666_280_ICC-IF_19011519_788.jpg
GTX15666_1510_WB_19011519_756.jpg
GTX15666_1511_WB_19011519_997.jpg
GTX15666_278_ICC-IF_19011519_420.jpg
GTX15666_279_ICC-IF_19011519_440.jpg
GTX15666_1998_ICC-IF_19011519_727.jpg

Cat No. GTX15666

Host Mouse
Clonality Monoclonal
Clone Name 10H11
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:250
IP Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atm Serine/Threonine Kinase,At1,Ata,Atc,Atd,Atdc,Ate,Tel1,Telo1,Atm

Cellular Localization

Cytoplasmic vesicle,Nucleus

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES
GTX15666_280_ICC-IF_19011519_788.jpg

GTX15666 ICC/IF Image

Orthogonal

ICC/IF analysis of 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX15666_1510_WB_19011519_756.jpg

GTX15666 WB Image

Orthogonal

WB analysis of 40 ug of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Dilution : 1:1000

GTX15666_1511_WB_19011519_997.jpg

GTX15666 WB Image

WB analysis of gamma-irradiated HeLa cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].

GTX15666_278_ICC-IF_19011519_420.jpg

GTX15666 ICC/IF Image

ICC/IF analysis of HeLa cells irradiated with UV for 4 hours using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes

GTX15666_279_ICC-IF_19011519_440.jpg

GTX15666 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

GTX15666_1998_ICC-IF_19011519_727.jpg

GTX15666 ICC/IF Image

ICC/IF analysis of HT-29 cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Left : Primary antibody
Right : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:50 and incubated overnight