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ATM (phospho Ser1981) antibody [HL1062]

Recombinant antibody Orthogonal
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in IHC (Paraffin sections) (IHC-P). GTX636086

Cat. No. GTX636086

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1062

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

351 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1981 of human ATM. The exact sequence is proprietary.

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2909941

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus,Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086

GTX636086 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636086

GTX636086 ICC/IF Image

Orthogonal

ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086

GTX636086 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATM (phospho Ser1981) antibody [HL1062] used in IHC (Paraffin sections) (IHC-P). GTX636086

GTX636086 IHC-P Image

ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yang JL et al. In Vivo 2021; 35 (5) : 2687-2696 Ouabain Induces DNA Damage in Human Osteosarcoma U-2 OS Cells and Alters the Expression of DNA Damage and DNA Repair-associated Proteins.
Application : WB
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations